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DeepPrime
Deep learning-based model that can predict the prime editing efficiency for all possible pegRNAs for a given target sequence
Published Paper
Publication
DeepPrime Pipeline
PE system: Choose model conditions closest to experimental parameters
Recommended: PE2max with optimized scaffold in HEK293T cells
Baseline: PE2 with conventional scaffold in HEK293T cells
---Fine-Tuned Models---
PE2_Conv with conventional scaffold in HEK293T cells
NRCH-PE2 with conventional scaffold in HEK293T cells
PE2 with optimized scaffold in HCT116 cells
PE2 with optimized scaffold in MDA-MD-231 cells
PE2max with optimized scaffold in HEK293T cells
PE2max with optimized scaffold in HeLa cells
PE2max with optimized scaffold in A549 cells
PE2max with optimized scaffold in DLD1 cells
NRCH-PE2max with optimized scaffold in HEK293T cells
PE4max with optimized scaffold in HEK293T cells
PE4max with optimized scaffold in A549 cells
PE4max with optimized scaffold in DLD1 cells
NRCH-PE4max with optimized scaffold in DLD1 cells
NRCH-PE4max with optimized scaffold in NIH3T3 cells
PE2max combined epegRNA with optimized scaffold in HEK293T cells
PE2max combined epegRNA with optimized scaffold in A549 cells
PE4max combined epegRNA with optimized scaffold in HEK293T cells
PE4max combined epegRNA with optimized scaffold in A549 cells
----------Off-target compatible----------
PE2_Conv with conventional scaffold in HEK293T cells
Edit type
Substitution
Insertion
Deletion
Edit length
1
2
3
Maximum Reverse Transcription Template (RTT) length in nucleotides (nt). Maximum= 40nt
Select Primer Binding Site (PBS) Range
MinPBS Length
MaxPBS Length
Analysis Flowchart
Interactive results table for the top 5 evaluated pegRNAs
DeepPrime Analysis: Input Sequence
Input Examples:
Substitution
Insertion
Deletion
Input Unedited Sequence(s) (121bp), comma-separated
Input Edited Sequence(s) (121bp), comma-separated
Submit
Reset
DeepPrime Analysis: Input ClinVar ID
Analysis Mode:
Model: Installation of ClinVar variant at target site
Therapy: Correction of ClinVar variant at target site
Enter
VCV
ID or Variant ID:
ex) VCV000009325.76 -or- 9325
Preview
Reset