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DeepSmallCas9
Select Cas9 enzyme
Multiple
selections are allowed.
sRGN3.1
SlugCas9
SaCas9
SauriCas9
Sa-SlugCas9
SaCas9-KKH
eSaCas9
efSaCas9
SauriCas9-KKH
SlugCas9-HF
SaCas9-HF
SaCas9-KKH-HF
St1Cas9
Nm1Cas9
enCjCas9
CjCas9
Nm2Cas9
Select reference genome
Single
selection is allowed.
Human (Ensembl GRCh38 v.104)
Mouse (RefSeq GRCm39 v.109)
None (w/o off-target activity prediction)
Potential off-target
Target with mismatch
0
1
2
3
4
5
Maximum
number of allowed mismatches.
Target with insertion or deletion (only applicable for
sRGN3.1
and
SlugCas9
)
0
1
Maximum
number of allowed insertions.
0
1
Maximum
number of allowed deletions.
Enter FASTA text
Enter CRISPR-Cas9 target sequences in FASTA format.
Enter a gene name or ID
Select
a species.
Human
Mouse
Enter
a gene name or ID.
Enter
a gene name or ID.
Upload a FASTA file
Upload a
.zip
file which contains single
.fasta
or
.fa
file.
[View an example input]
>LINC00478 (Chr21:17,959,604-17,960,104)
CAGACAGCATTAGAGTTGGCTTGAGAATTGCCGTACTTTGCTTCCCTTTGTATGTATTTC
TTGTATGCTGCCGAGTCACTGATGGCTAGCTCTGTCTGGCAAGTAATTCAAAAATGCTGT
TTATGTAGAAAGGAAAGGTAGGGACTTTACCACACTCTGTCATTAAAGGGAGCAATTGAA
GAACAAAGGAACTGAGTAAATACCTATATATTGCCTTTTGTGTTGCGAAACACTGTAGCA
CAAACACATTTGTGTTCAGCCAAATGTTTTACTTCCTTTTGTAATAACGCATATAGTAGG
TTGTCTCCACATATGTACAAGAATCCATATTTTATTTAAACGTATATAGTCAATTGTTCA
TATTTATAGGCTGCAAACATTTCTCAATCTCAAAGACTTTTACATATCCACTCCCACACA
GCTATTTGTTATTATTTTAAAAGTTCTTAAATTAAAAAAAAAAATAAAATATACTAATAT
CTCTGTTGGTTGATTTTATTA
[Download an example input]